Appearance

Purity determined by

White to yellowish lyophilized powder

Enzymatic Assay (ADH)

HPLC

NADH contents (anhydrous basis)

≥ 95%

≥ 95%

----

Water contents

< 8%

Na contents

6.5±1.5%

β-NAD (HPLC)

< 2%

Organic solvents(Methanol, ethanol,

Acetone, Isopropanol) determined by GC:

each<0.1%

Heavy metals (as Pb)

Arsenite (As)

≤ 5ppm

≤ 1ppm

NADH (A340, ε= 6.3 (1×mmol-1×cm-1)

≥ 90%

NADH (A260, ε= 14.3 (1×mmol-1×cm-1)

Ratio at pH 10

≥ 90%

A250/A260

A280/A260 A340A260

0.82±0.03

0.23±0.02

0.43±0.01

pH value in water(100mg/ml)

7.0-10.0

Total Bioburden

< 100 CFU/g

Coliform

< 100 CFU/g

Yeast and moulds

< 100 CFU/g

Salmonella

negative

Staph.aureus

negative

E.coli

negative